Detailed information of TA system TA118668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 88419..88978
• Replicon: plasmid p67_100
• Accession: NZ_CP035827
• Organism: Escherichia coli strain 67-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E3PPG0
• Locus tag: EXA05_RS25030
• Protein ID: WP_000421265.1
• Coordinates: 88703..88978 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E3PPG1
• Locus tag: EXA05_RS25025
• Protein ID: WP_001743519.1
• Coordinates: 88419..88703 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA05_RS25010	84606..85172	+	567	WP_000904908.1	recombinase family protein	-
EXA05_RS25015	85169..85582	+	414	WP_001046769.1	hypothetical protein	-
EXA05_RS25020	85914..87665	+	1752	WP_000038721.1	ATP-binding protein	-
EXA05_RS25025	88419..88703	+	285	WP_001743519.1	ribbon-helix-helix domain-containing protein	Antitoxin
EXA05_RS25030	88703..88978	+	276	WP_000421265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EXA05_RS25035	89141..90217	+	1077	WP_000610004.1	hypothetical protein	-
EXA05_RS25040	90468..90671	+	204	Protein_95	hypothetical protein	-
EXA05_RS25045	91280..91354	-	75	Protein_96	hypothetical protein	-
EXA05_RS25050	92768..93163	-	396	WP_000445942.1	plasmid partitioning/stability family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cfaD' / cfaE / cfaC / cfaB / cfaA / etpB / etpA	1..100283	100283
	-	flank	IS/Tn	-	-	81427..84561	3134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10742.45 Da
• Isoelectric Point: 9.9324

>T118668 WP_000421265.1 NZ_CP035827:88703-88978 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQASVILLTHPRMGEQLFQFELREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T118668 NZ_CP035827:88703-88978 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCATCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACTCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGTCTGTGGCATACGCGGGAAAACAGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 5.6546

>AT118668 WP_001743519.1 NZ_CP035827:88419-88703 [Escherichia coli]
MQMKNNCAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT118668 NZ_CP035827:88419-88703 [Escherichia coli]
ATGCAAATGAAAAACAATTGTGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAGCAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTAACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: E3PPG0

Antitoxin

• AlphaFold DB: E3PPG1