Detailed information of TA system TA118658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4489606..4490244
• Replicon: chromosome
• Accession: NZ_CP035826
• Organism: Escherichia coli strain 67-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: EXA05_RS21855
• Protein ID: WP_000813794.1
• Coordinates: 4489606..4489782 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EXA05_RS21860
• Protein ID: WP_001270286.1
• Coordinates: 4489828..4490244 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA05_RS21835 (4485225)	4485225..4486400	-	1176	WP_001236305.1	BenE family transporter YdcO	-
EXA05_RS21840 (4486492)	4486492..4487028	+	537	WP_000429169.1	DNA-binding transcriptional regulator SutR	-
EXA05_RS21845 (4487101)	4487101..4489062	+	1962	WP_001295654.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
EXA05_RS21850 (4489154)	4489154..4489384	-	231	WP_000494244.1	YncJ family protein	-
EXA05_RS21855 (4489606)	4489606..4489782	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
EXA05_RS21860 (4489828)	4489828..4490244	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
EXA05_RS21865 (4490323)	4490323..4491729	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
EXA05_RS21870 (4491974)	4491974..4493119	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
EXA05_RS21875 (4493137)	4493137..4494150	+	1014	WP_000220382.1	ABC transporter ATP-binding protein	-
EXA05_RS21880 (4494151)	4494151..4495092	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T118658 WP_000813794.1 NZ_CP035826:4489606-4489782 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T118658 NZ_CP035826:4489606-4489782 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT118658 WP_001270286.1 NZ_CP035826:4489828-4490244 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT118658 NZ_CP035826:4489828-4490244 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB