Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3413203..3413821 Replicon chromosome
Accession NZ_CP035826
Organism Escherichia coli strain 67-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EXA05_RS16525 Protein ID WP_001291435.1
Coordinates 3413203..3413421 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EXA05_RS16530 Protein ID WP_000344800.1
Coordinates 3413447..3413821 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA05_RS16490 (3408492) 3408492..3409064 + 573 WP_000779826.1 YbaY family lipoprotein -
EXA05_RS16495 (3409095) 3409095..3409406 - 312 WP_000409911.1 MGMT family protein -
EXA05_RS16505 (3409785) 3409785..3410138 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EXA05_RS16510 (3410180) 3410180..3411730 - 1551 WP_001484361.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EXA05_RS16515 (3411894) 3411894..3412364 - 471 WP_000136192.1 YlaC family protein -
EXA05_RS16520 (3412480) 3412480..3413031 - 552 WP_000102568.1 maltose O-acetyltransferase -
EXA05_RS16525 (3413203) 3413203..3413421 - 219 WP_001291435.1 HHA domain-containing protein Toxin
EXA05_RS16530 (3413447) 3413447..3413821 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EXA05_RS16535 (3414367) 3414367..3417516 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
EXA05_RS16540 (3417539) 3417539..3418732 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T118657 WP_001291435.1 NZ_CP035826:c3413421-3413203 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T118657 NZ_CP035826:c3413421-3413203 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT118657 WP_000344800.1 NZ_CP035826:c3413821-3413447 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT118657 NZ_CP035826:c3413821-3413447 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACTTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References