Detailed information of TA system TA118656

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3181518..3182197
• Replicon: chromosome
• Accession: NZ_CP035826
• Organism: Escherichia coli strain 67-1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: EXA05_RS15370
• Protein ID: WP_000854680.1
• Coordinates: 3181518..3181859 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: EXA05_RS15375
• Protein ID: WP_000070396.1
• Coordinates: 3181880..3182197 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA05_RS15355 (3177597)	3177597..3177944	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
EXA05_RS15360 (3177944)	3177944..3178621	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
EXA05_RS15365 (3179317)	3179317..3180423	+	1107	WP_000633074.1	SMEK domain-containing protein	-
EXA05_RS15370 (3181518)	3181518..3181859	-	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
EXA05_RS15375 (3181880)	3181880..3182197	-	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
EXA05_RS15380 (3182216)	3182216..3182437	-	222	WP_000691994.1	DUF987 domain-containing protein	-
EXA05_RS15385 (3182446)	3182446..3182922	-	477	WP_000811693.1	RadC family protein	-
EXA05_RS15390 (3182938)	3182938..3183396	-	459	WP_000211838.1	antirestriction protein	-
EXA05_RS15395 (3183494)	3183494..3183733	-	240	WP_000194654.1	DUF905 family protein	-
EXA05_RS15400 (3183810)	3183810..3184277	-	468	WP_001385283.1	protein YkfB	-
EXA05_RS15405 (3184300)	3184300..3184743	-	444	WP_000649865.1	hypothetical protein	-
EXA05_RS15410 (3184743)	3184743..3184979	-	237	WP_001144031.1	protein YpjK	-
EXA05_RS15415 (3185020)	3185020..3185721	-	702	WP_000189411.1	WYL domain-containing protein	-
EXA05_RS15420 (3185938)	3185938..3186759	-	822	WP_001385280.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T118656 WP_000854680.1 NZ_CP035826:c3181859-3181518 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T118656 NZ_CP035826:c3181859-3181518 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT118656 WP_000070396.1 NZ_CP035826:c3182197-3181880 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT118656 NZ_CP035826:c3182197-3181880 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7