118651

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) sanaTA/AbiEii-DUF6088
Location 1244253..1245588 Replicon chromosome
Accession NZ_CP035826
Organism Escherichia coli strain 67-1

Toxin (Protein)


Gene name sanaT Uniprot ID A0A0K4CDG2
Locus tag EXA05_RS06005 Protein ID WP_001440173.1
Coordinates 1244641..1245588 (+) Length 316 a.a.

Antitoxin (Protein)


Gene name sanaA Uniprot ID A0A0K6A7X2
Locus tag EXA05_RS06000 Protein ID WP_000208386.1
Coordinates 1244253..1244648 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA05_RS05955 (1239739) 1239739..1240224 + 486 WP_000213705.1 antirestriction protein -
EXA05_RS05960 (1240240) 1240240..1240716 + 477 WP_001186715.1 RadC family protein -
EXA05_RS05965 (1240785) 1240785..1241006 + 222 WP_000692345.1 DUF987 domain-containing protein -
EXA05_RS05970 (1241025) 1241025..1241669 + 645 WP_000086770.1 hypothetical protein -
EXA05_RS05975 (1241719) 1241719..1242087 + 369 WP_001285113.1 type IV toxin-antitoxin system YeeU family antitoxin -
EXA05_RS05980 (1242177) 1242177..1242554 + 378 WP_000854719.1 TA system toxin CbtA family protein -
EXA05_RS05985 (1242551) 1242551..1243039 + 489 WP_000761679.1 DUF5983 family protein -
EXA05_RS05990 (1243057) 1243057..1243254 + 198 WP_000839266.1 DUF957 domain-containing protein -
EXA05_RS05995 (1243339) 1243339..1244184 + 846 WP_001440175.1 DUF4942 domain-containing protein -
EXA05_RS06000 (1244253) 1244253..1244648 + 396 WP_000208386.1 DUF6088 family protein Antitoxin
EXA05_RS06005 (1244641) 1244641..1245588 + 948 WP_001440173.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
EXA05_RS06010 (1245943) 1245943..1246479 - 537 WP_000942792.1 GspM family type II secretion system protein YghD -
EXA05_RS06015 (1246481) 1246481..1247659 - 1179 WP_001384128.1 type II secretion system protein GspL -
EXA05_RS06020 (1247656) 1247656..1248633 - 978 WP_000633234.1 type II secretion system minor pseudopilin GspK -
EXA05_RS06025 (1248636) 1248636..1249235 - 600 WP_001255040.1 type II secretion system minor pseudopilin GspJ -
EXA05_RS06030 (1249232) 1249232..1249603 - 372 WP_000820111.1 type II secretion system minor pseudopilin GspI -
EXA05_RS06035 (1249600) 1249600..1250163 - 564 WP_001115154.1 type II secretion system minor pseudopilin GspH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island sul1 / qacE / ere(A) / dfrA5 / catA1 - 1173309..1245588 72279


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(36-302)

Antitoxin

Protein of unknown function DUF6088

(10-129)


Sequences

Toxin        


Download         Length: 316 a.a.        Molecular weight: 36284.66 Da        Isoelectric Point: 6.2532

>T118651 WP_001440173.1 NZ_CP035826:1244641-1245588 [Escherichia coli]
MPELTSLFPDVANALDIESVAIVEKDYFVVNLLKLLKEIKPETHTLVFAGGTALSKAGISLNRMSEDIDIKLVPTENFMQ
NGRNRKRKIRKEIVQIITDVLHNSDIFSLDNENARITRDEYRYNEIPVRYPQTFAQAPCLRPFIKLELMETTLLEHPESR
DIYSLVTELTGNGTPVTAFPCATILSTQAEKLISMMRRTAILLRNPEQQDDESLVRHIYDNYCIVREKGVNVPVLKNFVQ
ICIQQDIERYGNQHPQFRNSPVDELKKGLDALRDDPVHKLRFQRFVKPMVFGNTQIDWEEAYSCFRQTALSVLTN

Download         Length: 948 bp

>T118651 NZ_CP035826:1244641-1245588 [Escherichia coli]
ATGCCTGAATTAACATCATTATTTCCTGACGTTGCCAACGCACTGGATATTGAATCTGTCGCCATCGTCGAAAAAGATTA
TTTTGTGGTTAATCTTTTAAAGTTACTAAAAGAAATAAAACCAGAAACACATACTCTCGTGTTTGCAGGAGGTACCGCAT
TGTCAAAAGCTGGTATCTCATTGAACAGAATGTCAGAAGATATTGATATAAAGCTCGTGCCAACAGAGAACTTTATGCAA
AATGGCAGAAACAGGAAAAGAAAAATTCGCAAGGAAATCGTGCAAATAATCACGGATGTTCTTCATAATTCTGACATTTT
CAGCCTTGATAATGAAAACGCCCGTATAACACGTGATGAGTATCGTTATAACGAGATCCCCGTACGTTATCCCCAGACAT
TTGCCCAGGCCCCCTGTCTTCGTCCATTTATTAAGCTGGAGTTGATGGAAACCACTCTGCTGGAACACCCGGAAAGCAGA
GATATTTACTCACTCGTCACGGAGTTAACGGGTAATGGCACACCTGTAACAGCATTCCCCTGTGCCACCATTTTATCCAC
TCAGGCTGAAAAGCTCATATCCATGATGCGAAGAACAGCCATACTTCTTCGCAATCCCGAACAACAGGATGATGAGTCCC
TGGTACGCCACATTTACGACAATTATTGCATTGTCAGGGAAAAAGGTGTGAATGTTCCAGTACTGAAAAATTTTGTTCAG
ATATGTATTCAACAGGACATTGAGCGCTATGGTAACCAGCACCCACAATTTCGTAACTCACCAGTTGATGAACTTAAGAA
AGGGCTGGATGCACTCCGGGATGATCCTGTACACAAATTGCGTTTTCAACGATTTGTTAAACCGATGGTATTTGGTAATA
CACAGATTGACTGGGAAGAAGCCTACTCCTGTTTCAGACAAACAGCGTTGAGTGTTCTGACCAACTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14639.00 Da        Isoelectric Point: 11.0607

>AT118651 WP_000208386.1 NZ_CP035826:1244253-1244648 [Escherichia coli]
MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEGKLMKVGYGVYTKARRNVITGKIMPAAPGGSAAVITE
ALDRLNIRYSLIGATECYNKGQSTQIPVSPEFKTPPRFKRVLVVGKSKINA

Download         Length: 396 bp

>AT118651 NZ_CP035826:1244253-1244648 [Escherichia coli]
ATGACTACAAAAGACCGTATTCAGTCCCGACTGAACCGTTCAAAGCGTTATGTGTTCACCCGGGATGACTTCAGGGATAT
CGCTGGCTATGATCAGGTTGGCAGAGCTCTTCGTACGCTGGTAAACGAAGGTAAGTTGATGAAAGTGGGCTATGGTGTTT
ACACCAAAGCCCGTCGCAACGTTATTACAGGAAAAATCATGCCTGCAGCACCTGGTGGCTCTGCCGCTGTAATCACCGAA
GCCCTGGATCGTTTGAATATCAGATACAGCTTGATCGGCGCAACAGAATGCTACAATAAAGGTCAGTCCACACAGATTCC
GGTATCTCCGGAGTTCAAAACACCTCCTCGCTTTAAAAGAGTGTTAGTTGTAGGGAAAAGTAAAATCAATGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4CDG2

Antitoxin

Source ID Structure
AlphaFold DB A0A0K6A7X2

References