Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1097185..1097839 Replicon chromosome
Accession NZ_CP035826
Organism Escherichia coli strain 67-1

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag EXA05_RS05230 Protein ID WP_000244781.1
Coordinates 1097185..1097592 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EXA05_RS05235 Protein ID WP_000354046.1
Coordinates 1097573..1097839 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA05_RS05210 (1093142) 1093142..1094875 - 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -
EXA05_RS05215 (1094881) 1094881..1095591 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EXA05_RS05220 (1095616) 1095616..1096512 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EXA05_RS05225 (1096624) 1096624..1097145 + 522 WP_001055874.1 flavodoxin FldB -
EXA05_RS05230 (1097185) 1097185..1097592 - 408 WP_000244781.1 protein YgfX Toxin
EXA05_RS05235 (1097573) 1097573..1097839 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EXA05_RS05240 (1098082) 1098082..1099062 + 981 WP_000886083.1 tRNA-modifying protein YgfZ -
EXA05_RS05245 (1099258) 1099258..1099917 - 660 WP_000250274.1 hemolysin III family protein -
EXA05_RS05250 (1100081) 1100081..1100392 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
EXA05_RS05255 (1100437) 1100437..1101870 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
EXA05_RS05260 (1101927) 1101927..1102670 - 744 WP_000951939.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T118649 WP_000244781.1 NZ_CP035826:c1097592-1097185 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118649 NZ_CP035826:c1097592-1097185 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT118649 WP_000354046.1 NZ_CP035826:c1097839-1097573 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118649 NZ_CP035826:c1097839-1097573 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References