Detailed information of TA system TA118646

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 86113..86948
• Replicon: chromosome
• Accession: NZ_CP035826
• Organism: Escherichia coli strain 67-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1U9SHU4
• Locus tag: EXA05_RS00365
• Protein ID: WP_000854752.1
• Coordinates: 86571..86948 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: EXA05_RS00360
• Protein ID: WP_001278232.1
• Coordinates: 86113..86481 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA05_RS00330 (82949)	82949..83404	+	456	WP_000581504.1	IrmA family protein	-
EXA05_RS00335 (83483)	83483..83716	+	234	WP_001119729.1	DUF905 family protein	-
EXA05_RS00340 (83816)	83816..84634	+	819	WP_001234620.1	DUF932 domain-containing protein	-
EXA05_RS00345 (84689)	84689..85174	+	486	WP_000849582.1	antirestriction protein	-
EXA05_RS00350 (85190)	85190..85666	+	477	WP_001186774.1	RadC family protein	-
EXA05_RS00355 (85729)	85729..85950	+	222	WP_000692318.1	DUF987 domain-containing protein	-
EXA05_RS00360 (86113)	86113..86481	+	369	WP_001278232.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
EXA05_RS00365 (86571)	86571..86948	+	378	WP_000854752.1	TA system toxin CbtA family protein	Toxin
EXA05_RS00370 (86945)	86945..87094	+	150	Protein_68	DUF5983 family protein	-
EXA05_RS00375 (87194)	87194..87274	+	81	Protein_69	hypothetical protein	-
EXA05_RS00380 (88090)	88090..88461	+	372	WP_001295631.1	IS110 family transposase	-
EXA05_RS00385 (88620)	88620..89099	-	480	Protein_71	IS3 family transposase	-
EXA05_RS00390 (89183)	89183..90691	-	1509	WP_001474883.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14076.08 Da
• Isoelectric Point: 8.2905

>T118646 WP_000854752.1 NZ_CP035826:86571-86948 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T118646 NZ_CP035826:86571-86948 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCCCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT118646 WP_001278232.1 NZ_CP035826:86113-86481 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT118646 NZ_CP035826:86113-86481 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1U9SHU4

Antitoxin

• AlphaFold DB: E3PJ72