Detailed information of TA system TA118645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 55165..55724
• Replicon: plasmid pWS4437A1_99
• Accession: NZ_CP035823
• Organism: Escherichia coli strain WS4437A-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E3PPG0
• Locus tag: EXA06_RS23715
• Protein ID: WP_000421265.1
• Coordinates: 55165..55440 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E3PPG1
• Locus tag: EXA06_RS23720
• Protein ID: WP_001743519.1
• Coordinates: 55440..55724 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA06_RS23695	50980..51375	+	396	WP_000445942.1	plasmid partitioning/stability family protein	-
EXA06_RS23700	52789..52863	+	75	Protein_64	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
EXA06_RS23705	53472..53675	-	204	Protein_65	hypothetical protein	-
EXA06_RS23710	53926..55002	-	1077	WP_000610004.1	hypothetical protein	-
EXA06_RS23715	55165..55440	-	276	WP_000421265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EXA06_RS23720	55440..55724	-	285	WP_001743519.1	ribbon-helix-helix domain-containing protein	Antitoxin
EXA06_RS23725	56478..58229	-	1752	WP_000038721.1	ATP-binding protein	-
EXA06_RS24750	58561..58974	-	414	WP_001046769.1	hypothetical protein	-
EXA06_RS23735	58971..59537	-	567	WP_000904908.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	etpB / cfaA / cfaB / cfaC / cfaE / cfaD'	1..99306	99306
	-	flank	IS/Tn	-	-	59582..62716	3134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10742.45 Da
• Isoelectric Point: 9.9324

>T118645 WP_000421265.1 NZ_CP035823:c55440-55165 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQASVILLTHPRMGEQLFQFELREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T118645 NZ_CP035823:c55440-55165 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCATCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACTCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGTCTGTGGCATACGCGGGAAAACAGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 5.6546

>AT118645 WP_001743519.1 NZ_CP035823:c55724-55440 [Escherichia coli]
MQMKNNCAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT118645 NZ_CP035823:c55724-55440 [Escherichia coli]
ATGCAAATGAAAAACAATTGTGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAGCAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTAACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: E3PPG0

Antitoxin

• AlphaFold DB: E3PPG1