Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4768662..4769280 Replicon chromosome
Accession NZ_CP035822
Organism Escherichia coli strain WS4437A-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EXA06_RS23090 Protein ID WP_001291435.1
Coordinates 4768662..4768880 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EXA06_RS23095 Protein ID WP_000344800.1
Coordinates 4768906..4769280 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA06_RS23055 (4763951) 4763951..4764523 + 573 WP_000779831.1 YbaY family lipoprotein -
EXA06_RS23060 (4764554) 4764554..4764865 - 312 WP_000409911.1 MGMT family protein -
EXA06_RS23070 (4765244) 4765244..4765597 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EXA06_RS23075 (4765639) 4765639..4767189 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EXA06_RS23080 (4767353) 4767353..4767823 - 471 WP_000136192.1 YlaC family protein -
EXA06_RS23085 (4767939) 4767939..4768490 - 552 WP_000102564.1 maltose O-acetyltransferase -
EXA06_RS23090 (4768662) 4768662..4768880 - 219 WP_001291435.1 HHA domain-containing protein Toxin
EXA06_RS23095 (4768906) 4768906..4769280 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EXA06_RS23100 (4769826) 4769826..4772975 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
EXA06_RS23105 (4772998) 4772998..4774191 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T118644 WP_001291435.1 NZ_CP035822:c4768880-4768662 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T118644 NZ_CP035822:c4768880-4768662 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT118644 WP_000344800.1 NZ_CP035822:c4769280-4768906 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT118644 NZ_CP035822:c4769280-4768906 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References