Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2549718..2550372 Replicon chromosome
Accession NZ_CP035822
Organism Escherichia coli strain WS4437A-1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag EXA06_RS12410 Protein ID WP_000244777.1
Coordinates 2549718..2550125 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EXA06_RS12415 Protein ID WP_000354046.1
Coordinates 2550106..2550372 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA06_RS12390 (2545675) 2545675..2547408 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
EXA06_RS12395 (2547414) 2547414..2548124 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EXA06_RS12400 (2548149) 2548149..2549045 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EXA06_RS12405 (2549157) 2549157..2549678 + 522 WP_001055874.1 flavodoxin FldB -
EXA06_RS12410 (2549718) 2549718..2550125 - 408 WP_000244777.1 protein YgfX Toxin
EXA06_RS12415 (2550106) 2550106..2550372 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EXA06_RS12420 (2550615) 2550615..2551595 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EXA06_RS12425 (2551791) 2551791..2552450 - 660 WP_000250274.1 hemolysin III family protein -
EXA06_RS12430 (2552614) 2552614..2552925 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
EXA06_RS12435 (2552970) 2552970..2554403 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
EXA06_RS12440 (2554460) 2554460..2555203 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T118636 WP_000244777.1 NZ_CP035822:c2550125-2549718 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118636 NZ_CP035822:c2550125-2549718 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT118636 WP_000354046.1 NZ_CP035822:c2550372-2550106 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118636 NZ_CP035822:c2550372-2550106 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References