Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 947488..948126 Replicon chromosome
Accession NZ_CP035822
Organism Escherichia coli strain WS4437A-1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag EXA06_RS04690 Protein ID WP_000813794.1
Coordinates 947488..947664 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EXA06_RS04695 Protein ID WP_001270286.1
Coordinates 947710..948126 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA06_RS04670 (943107) 943107..944282 - 1176 WP_001236265.1 BenE family transporter YdcO -
EXA06_RS04675 (944374) 944374..944910 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
EXA06_RS04680 (944983) 944983..946944 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
EXA06_RS04685 (947036) 947036..947266 - 231 WP_000494244.1 YncJ family protein -
EXA06_RS04690 (947488) 947488..947664 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
EXA06_RS04695 (947710) 947710..948126 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
EXA06_RS04700 (948205) 948205..949611 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
EXA06_RS04705 (949856) 949856..951001 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
EXA06_RS04710 (951019) 951019..952032 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
EXA06_RS04715 (952033) 952033..952974 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T118623 WP_000813794.1 NZ_CP035822:947488-947664 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T118623 NZ_CP035822:947488-947664 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT118623 WP_001270286.1 NZ_CP035822:947710-948126 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT118623 NZ_CP035822:947710-948126 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References