Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 81680..82227 Replicon plasmid pWS3294A_133
Accession NZ_CP035820
Organism Escherichia coli strain WS3294A

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag EXA07_RS24685 Protein ID WP_001384452.1
Coordinates 81680..81958 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A2I2C2Y5
Locus tag EXA07_RS24690 Protein ID WP_000079948.1
Coordinates 81958..82227 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA07_RS24665 (78175) 78175..78696 - 522 Protein_91 hypothetical protein -
EXA07_RS24670 (79050) 79050..79652 + 603 WP_000517689.1 ProQ/FINO family protein -
EXA07_RS24675 (80187) 80187..80669 - 483 WP_032182006.1 hypothetical protein -
EXA07_RS24680 (80786) 80786..81634 - 849 WP_001057994.1 exonuclease domain-containing protein -
EXA07_RS24685 (81680) 81680..81958 - 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
EXA07_RS24690 (81958) 81958..82227 - 270 WP_000079948.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
EXA07_RS24695 (83142) 83142..83999 - 858 WP_000130978.1 incFII family plasmid replication initiator RepA -
EXA07_RS25760 (83992) 83992..84039 - 48 WP_235588261.1 hypothetical protein -
EXA07_RS25765 (84030) 84030..84266 + 237 WP_235588260.1 replication protein RepA -
EXA07_RS24705 (84302) 84302..84559 - 258 WP_000083823.1 replication regulatory protein RepA -
EXA07_RS24710 (85066) 85066..85248 + 183 WP_001393357.1 hypothetical protein -
EXA07_RS24715 (85473) 85473..86204 - 732 WP_001071488.1 type-F conjugative transfer system pilin acetylase TraX -
EXA07_RS24720 (86201) 86201..86767 - 567 WP_000469207.1 DUF2726 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - etpA / etpB / cfaA / csaC / csaE 1..133987 133987


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T118622 WP_001384452.1 NZ_CP035820:c81958-81680 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T118622 NZ_CP035820:c81958-81680 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10251.64 Da        Isoelectric Point: 10.1734

>AT118622 WP_000079948.1 NZ_CP035820:c82227-81958 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLKRGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT118622 NZ_CP035820:c82227-81958 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCAAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A2I2C2Y5

References