Detailed information of TA system TA118621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 32357..33000
• Replicon: plasmid pWS3294A_133
• Accession: NZ_CP035820
• Organism: Escherichia coli strain WS3294A

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: EXA07_RS24395
• Protein ID: WP_001356337.1
• Coordinates: 32357..32773 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: EXA07_RS24400
• Protein ID: WP_024220872.1
• Coordinates: 32770..33000 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA07_RS24355 (27633)	27633..27980	-	348	WP_000681610.1	type II toxin-antitoxin system HicB family antitoxin	-
EXA07_RS24360 (27977)	27977..28225	-	249	WP_000513531.1	type II toxin-antitoxin system HicA family toxin	-
EXA07_RS24365 (28690)	28690..29001	-	312	WP_000729511.1	hypothetical protein	-
EXA07_RS24370 (29043)	29043..29740	+	698	Protein_30	IS1 family transposase	-
EXA07_RS24375 (30056)	30056..30205	+	150	Protein_31	transposase	-
EXA07_RS24380 (30214)	30214..30489	-	276	Protein_32	integrase core domain-containing protein	-
EXA07_RS24385 (30542)	30542..31755	+	1214	WP_187536092.1	IS3 family transposase	-
EXA07_RS25750 (31797)	31797..31931	-	135	Protein_34	hypothetical protein	-
EXA07_RS24390 (32043)	32043..32390	+	348	Protein_35	helix-turn-helix domain-containing protein	-
EXA07_RS24395 (32357)	32357..32773	-	417	WP_001356337.1	type II toxin-antitoxin system VapC family toxin	Toxin
EXA07_RS24400 (32770)	32770..33000	-	231	WP_024220872.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
EXA07_RS24405 (33281)	33281..34300	-	1020	Protein_38	IS110 family transposase	-
EXA07_RS24410 (34926)	34926..35339	+	414	WP_000465050.1	hypothetical protein	-
EXA07_RS24415 (35341)	35341..35823	+	483	Protein_40	site-specific integrase	-
EXA07_RS24425 (37154)	37154..37459	+	306	Protein_42	site-specific integrase	-
EXA07_RS24430 (37597)	37597..37872	-	276	WP_000239529.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	etpA / etpB / cfaA / csaC / csaE	1..133987	133987
	-	inside	IScluster/Tn	-	-	25186..41983	16797

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15059.51 Da
• Isoelectric Point: 7.7814

>T118621 WP_001356337.1 NZ_CP035820:c32773-32357 [Escherichia coli]
MKKTYMLDTCICSFIMREQPEAVLNRLEQTVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLIDAFCARLDAVLPWDRT
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTSNVREFARVPGLVLEDWVN

Nucleotide

• Length: 417 bp

>T118621 NZ_CP035820:c32773-32357 [Escherichia coli]
ATGAAGAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCAGAAGCTGTGCTGAATCGCCT
GGAGCAGACGGTGCTGCGCGGTCACCGTATCGTGGTCTCAGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAAGCCTCACCGCGTCACGTACAGTTGATTGACGCGTTCTGCGCCCGGCTCGATGCCGTCCTGCCCTGGGACCGCACC
GCGGTGGATGCCACCACAGAGATTAAGGTGGCCCTGCGCCTGGCCGGCACGCCGATCGGTCCGAACGACACCGCCATTGC
CGGACACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAGTAATGTGAGAGAGTTTGCACGGGTGCCAGGCCTGGTGCTGG
AAGACTGGGTTAACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8792.91 Da
• Isoelectric Point: 4.4992

>AT118621 WP_024220872.1 NZ_CP035820:c33000-32770 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPVRPSWGSFAQLEKADPDFMAERDDVVTDEGRFEL

Nucleotide

• Length: 231 bp

>AT118621 NZ_CP035820:c33000-32770 [Escherichia coli]
ATGAGAACAGTATCCATTTTTAAAAACGGCAATAACCGTGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATCGTCCGGGAAGGGGACCGCATCATCCTGCGTCCCGTCCGGCCGAGCTGGGGCTCGTTCGCGCAGCTTG
AAAAGGCCGATCCAGACTTTATGGCAGAGCGCGATGACGTTGTCACCGACGAAGGACGCTTTGAACTATGA