Detailed information of TA system TA118620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 27633..28225
• Replicon: plasmid pWS3294A_133
• Accession: NZ_CP035820
• Organism: Escherichia coli strain WS3294A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EXA07_RS24360
• Protein ID: WP_000513531.1
• Coordinates: 27977..28225 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EXA07_RS24355
• Protein ID: WP_000681610.1
• Coordinates: 27633..27980 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA07_RS24335 (23173)	23173..24984	-	1812	WP_000260520.1	two-partner secretion system transporter EtpB	-
EXA07_RS24340 (25186)	25186..25788	-	603	Protein_24	DDE-type integrase/transposase/recombinase	-
EXA07_RS24345 (25870)	25870..26235	+	366	WP_000124139.1	hypothetical protein	-
EXA07_RS24350 (26235)	26235..27406	+	1172	Protein_26	IS91 family transposase	-
EXA07_RS24355 (27633)	27633..27980	-	348	WP_000681610.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EXA07_RS24360 (27977)	27977..28225	-	249	WP_000513531.1	type II toxin-antitoxin system HicA family toxin	Toxin
EXA07_RS24365 (28690)	28690..29001	-	312	WP_000729511.1	hypothetical protein	-
EXA07_RS24370 (29043)	29043..29740	+	698	Protein_30	IS1 family transposase	-
EXA07_RS24375 (30056)	30056..30205	+	150	Protein_31	transposase	-
EXA07_RS24380 (30214)	30214..30489	-	276	Protein_32	integrase core domain-containing protein	-
EXA07_RS24385 (30542)	30542..31755	+	1214	WP_187536092.1	IS3 family transposase	-
EXA07_RS25750 (31797)	31797..31931	-	135	Protein_34	hypothetical protein	-
EXA07_RS24390 (32043)	32043..32390	+	348	Protein_35	helix-turn-helix domain-containing protein	-
EXA07_RS24395 (32357)	32357..32773	-	417	WP_001356337.1	type II toxin-antitoxin system VapC family toxin	-
EXA07_RS24400 (32770)	32770..33000	-	231	WP_024220872.1	type II toxin-antitoxin system VapB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	etpA / etpB / cfaA / csaC / csaE	1..133987	133987
	-	inside	IScluster/Tn	-	-	25186..41983	16797

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-80)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9298.86 Da
• Isoelectric Point: 9.9409

>T118620 WP_000513531.1 NZ_CP035820:c28225-27977 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKDMQGSRVRFFNAEINHTILMHCPHPESYIKGGTLKTIKQNLNEAG
AL

Nucleotide

• Length: 249 bp

>T118620 NZ_CP035820:c28225-27977 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTATT
TTCTTCTCTAGGATATGTCAAAAAAGACATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATTGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAACTATTAAACAGAACTTGAACGAGGCTGGG
GCTTTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13152.97 Da
• Isoelectric Point: 4.9966

>AT118620 WP_000681610.1 NZ_CP035820:c27980-27633 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAKTLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVGNHRAGA

Nucleotide

• Length: 348 bp

>AT118620 NZ_CP035820:c27980-27633 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAAAAACATTAGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGCCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCGCTGGAAAAAGA
AGTCGGTAATCATCGCGCAGGGGCTTAA