Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4033902..4034520 Replicon chromosome
Accession NZ_CP035819
Organism Escherichia coli strain WS3294A

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EXA07_RS19345 Protein ID WP_001291435.1
Coordinates 4033902..4034120 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EXA07_RS19350 Protein ID WP_000344800.1
Coordinates 4034146..4034520 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA07_RS19310 (4029191) 4029191..4029763 + 573 WP_000779831.1 YbaY family lipoprotein -
EXA07_RS19315 (4029794) 4029794..4030105 - 312 WP_000409911.1 MGMT family protein -
EXA07_RS19325 (4030484) 4030484..4030837 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EXA07_RS19330 (4030879) 4030879..4032429 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EXA07_RS19335 (4032593) 4032593..4033063 - 471 WP_000136192.1 YlaC family protein -
EXA07_RS19340 (4033179) 4033179..4033730 - 552 WP_000102568.1 maltose O-acetyltransferase -
EXA07_RS19345 (4033902) 4033902..4034120 - 219 WP_001291435.1 HHA domain-containing protein Toxin
EXA07_RS19350 (4034146) 4034146..4034520 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EXA07_RS19355 (4035066) 4035066..4038215 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
EXA07_RS19360 (4038238) 4038238..4039431 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T118619 WP_001291435.1 NZ_CP035819:c4034120-4033902 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T118619 NZ_CP035819:c4034120-4033902 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT118619 WP_000344800.1 NZ_CP035819:c4034520-4034146 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT118619 NZ_CP035819:c4034520-4034146 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References