Detailed information of TA system TA118618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3999594..4000431
• Replicon: chromosome
• Accession: NZ_CP035819
• Organism: Escherichia coli strain WS3294A

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: EXA07_RS19175
• Protein ID: WP_096960763.1
• Coordinates: 3999594..4000136 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: EXA07_RS19180
• Protein ID: WP_001297137.1
• Coordinates: 4000120..4000431 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA07_RS19155 (3995133)	3995133..3996044	-	912	WP_000705853.1	2-dehydropantoate 2-reductase	-
EXA07_RS19160 (3996212)	3996212..3996703	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
EXA07_RS19165 (3996831)	3996831..3998195	-	1365	WP_001000978.1	MFS transporter	-
EXA07_RS19170 (3998603)	3998603..3999538	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
EXA07_RS19175 (3999594)	3999594..4000136	-	543	WP_096960763.1	GNAT family N-acetyltransferase	Toxin
EXA07_RS19180 (4000120)	4000120..4000431	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
EXA07_RS19185 (4000616)	4000616..4001506	-	891	WP_000971336.1	heme o synthase	-
EXA07_RS19190 (4001518)	4001518..4001847	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
EXA07_RS19195 (4001847)	4001847..4002461	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
EXA07_RS19200 (4002451)	4002451..4004442	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
EXA07_RS19205 (4004464)	4004464..4005411	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (67-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19776.97 Da
• Isoelectric Point: 8.3395

>T118618 WP_096960763.1 NZ_CP035819:c4000136-3999594 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRASGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T118618 NZ_CP035819:c4000136-3999594 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGCTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT118618 WP_001297137.1 NZ_CP035819:c4000431-4000120 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT118618 NZ_CP035819:c4000431-4000120 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3