Detailed information of TA system TA118616

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3397460..3398292
• Replicon: chromosome
• Accession: NZ_CP035819
• Organism: Escherichia coli strain WS3294A

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: EXA07_RS16360
• Protein ID: WP_000854765.1
• Coordinates: 3397918..3398292 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: EXA07_RS16355
• Protein ID: WP_001295723.1
• Coordinates: 3397460..3397828 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA07_RS16325 (3394296)	3394296..3394751	+	456	WP_000581504.1	IrmA family protein	-
EXA07_RS16330 (3394830)	3394830..3395063	+	234	WP_001119729.1	DUF905 family protein	-
EXA07_RS16335 (3395163)	3395163..3395981	+	819	WP_001234620.1	DUF932 domain-containing protein	-
EXA07_RS16340 (3396036)	3396036..3396521	+	486	WP_000849588.1	antirestriction protein	-
EXA07_RS16345 (3396537)	3396537..3397013	+	477	WP_001186774.1	RadC family protein	-
EXA07_RS16350 (3397076)	3397076..3397297	+	222	WP_000692341.1	DUF987 domain-containing protein	-
EXA07_RS16355 (3397460)	3397460..3397828	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EXA07_RS16360 (3397918)	3397918..3398292	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
EXA07_RS16365 (3398289)	3398289..3398780	+	492	WP_000976842.1	DUF5983 family protein	-
EXA07_RS16370 (3398797)	3398797..3398973	+	177	WP_000839290.1	DUF957 domain-containing protein	-
EXA07_RS16375 (3399079)	3399079..3399228	+	150	Protein_3193	hypothetical protein	-
EXA07_RS16385 (3400359)	3400359..3400529	+	171	Protein_3195	fimbrial protein	-
EXA07_RS16390 (3400703)	3400703..3402046	-	1344	WP_000558251.1	gluconate permease GntP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T118616 WP_000854765.1 NZ_CP035819:3397918-3398292 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T118616 NZ_CP035819:3397918-3398292 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT118616 WP_001295723.1 NZ_CP035819:3397460-3397828 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT118616 NZ_CP035819:3397460-3397828 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52