Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3204359..3204856 Replicon chromosome
Accession NZ_CP035819
Organism Escherichia coli strain WS3294A

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EXA07_RS15410 Protein ID WP_001173343.1
Coordinates 3204683..3204856 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EXA07_RS15405 Protein ID WP_000398619.1
Coordinates 3204359..3204655 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA07_RS15385 (3201090) 3201090..3201809 - 720 WP_000611288.1 two-component system response regulator DcuR -
EXA07_RS15390 (3201806) 3201806..3203437 - 1632 WP_001216461.1 sensor histidine kinase -
EXA07_RS15395 (3203618) 3203618..3203848 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
EXA07_RS15400 (3203860) 3203860..3204132 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
EXA07_RS15405 (3204359) 3204359..3204655 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EXA07_RS15410 (3204683) 3204683..3204856 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EXA07_RS15415 (3204975) 3204975..3206492 - 1518 WP_001295074.1 lysine--tRNA ligase -
EXA07_RS15420 (3206729) 3206729..3208186 - 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T118615 WP_001173343.1 NZ_CP035819:3204683-3204856 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T118615 NZ_CP035819:3204683-3204856 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT118615 WP_000398619.1 NZ_CP035819:3204359-3204655 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT118615 NZ_CP035819:3204359-3204655 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References