Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2423132..2423718 Replicon chromosome
Accession NZ_CP035819
Organism Escherichia coli strain WS3294A

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag EXA07_RS11880 Protein ID WP_042014162.1
Coordinates 2423488..2423718 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag EXA07_RS11875 Protein ID WP_000593555.1
Coordinates 2423132..2423491 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA07_RS11850 (2419264) 2419264..2420208 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
EXA07_RS11855 (2420205) 2420205..2421038 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
EXA07_RS11860 (2421038) 2421038..2421802 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
EXA07_RS11865 (2421799) 2421799..2422605 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
EXA07_RS11870 (2422611) 2422611..2423012 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
EXA07_RS11875 (2423132) 2423132..2423491 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EXA07_RS11880 (2423488) 2423488..2423718 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
EXA07_RS11885 (2423822) 2423822..2424946 - 1125 WP_001314210.1 ABC transporter permease -
EXA07_RS11890 (2424946) 2424946..2427681 - 2736 WP_216350963.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T118613 WP_042014162.1 NZ_CP035819:c2423718-2423488 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T118613 NZ_CP035819:c2423718-2423488 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT118613 WP_000593555.1 NZ_CP035819:c2423491-2423132 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT118613 NZ_CP035819:c2423491-2423132 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References