Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1827389..1828043 Replicon chromosome
Accession NZ_CP035819
Organism Escherichia coli strain WS3294A

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag EXA07_RS08865 Protein ID WP_000244772.1
Coordinates 1827389..1827796 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EXA07_RS08870 Protein ID WP_000354046.1
Coordinates 1827777..1828043 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA07_RS08845 (1823346) 1823346..1825079 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
EXA07_RS08850 (1825085) 1825085..1825795 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EXA07_RS08855 (1825820) 1825820..1826716 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EXA07_RS08860 (1826828) 1826828..1827349 + 522 WP_001055874.1 flavodoxin FldB -
EXA07_RS08865 (1827389) 1827389..1827796 - 408 WP_000244772.1 protein YgfX Toxin
EXA07_RS08870 (1827777) 1827777..1828043 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EXA07_RS08875 (1828286) 1828286..1829266 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EXA07_RS08880 (1829343) 1829343..1830002 - 660 WP_000250274.1 hemolysin III family protein -
EXA07_RS08885 (1830166) 1830166..1830477 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
EXA07_RS08890 (1830522) 1830522..1831955 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
EXA07_RS08895 (1832012) 1832012..1832755 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T118610 WP_000244772.1 NZ_CP035819:c1827796-1827389 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118610 NZ_CP035819:c1827796-1827389 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT118610 WP_000354046.1 NZ_CP035819:c1828043-1827777 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118610 NZ_CP035819:c1828043-1827777 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References