Detailed information of TA system TA118608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1371433..1372058
• Replicon: chromosome
• Accession: NZ_CP035819
• Organism: Escherichia coli strain WS3294A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EXA07_RS06695
• Protein ID: WP_000911330.1
• Coordinates: 1371433..1371831 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EXA07_RS06700
• Protein ID: WP_000450524.1
• Coordinates: 1371831..1372058 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA07_RS06675 (1367311)	1367311..1367511	+	201	WP_000383836.1	YpfN family protein	-
EXA07_RS06680 (1367621)	1367621..1368319	-	699	WP_000679823.1	esterase	-
EXA07_RS06685 (1368393)	1368393..1370408	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
EXA07_RS06690 (1370423)	1370423..1371286	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
EXA07_RS06695 (1371433)	1371433..1371831	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
EXA07_RS06700 (1371831)	1371831..1372058	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EXA07_RS06705 (1372212)	1372212..1372925	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EXA07_RS06710 (1373138)	1373138..1374172	-	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
EXA07_RS06715 (1374189)	1374189..1375067	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EXA07_RS06720 (1375213)	1375213..1375785	+	573	WP_000176191.1	glycine cleavage system transcriptional repressor	-
EXA07_RS06725 (1375785)	1375785..1376255	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T118608 WP_000911330.1 NZ_CP035819:c1371831-1371433 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T118608 NZ_CP035819:c1371831-1371433 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT118608 WP_000450524.1 NZ_CP035819:c1372058-1371831 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT118608 NZ_CP035819:c1372058-1371831 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33