Detailed information of TA system TA118606

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 812838..813670
• Replicon: chromosome
• Accession: NZ_CP035819
• Organism: Escherichia coli strain WS3294A

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: EXA07_RS04040
• Protein ID: WP_000854762.1
• Coordinates: 813296..813670 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: EXA07_RS04035
• Protein ID: WP_001295723.1
• Coordinates: 812838..813206 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA07_RS04010 (809953)	809953..810148	+	196	Protein_791	DUF905 family protein	-
EXA07_RS04015 (810266)	810266..811084	+	819	WP_001234719.1	DUF932 domain-containing protein	-
EXA07_RS04020 (811426)	811426..811899	+	474	WP_000855059.1	antirestriction protein	-
EXA07_RS04025 (811915)	811915..812391	+	477	WP_001186774.1	RadC family protein	-
EXA07_RS04030 (812454)	812454..812675	+	222	WP_000692341.1	DUF987 domain-containing protein	-
EXA07_RS04035 (812838)	812838..813206	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EXA07_RS04040 (813296)	813296..813670	+	375	WP_000854762.1	TA system toxin CbtA family protein	Toxin
EXA07_RS04045 (813667)	813667..813819	+	153	Protein_798	DUF5983 family protein	-
EXA07_RS04050 (813919)	813919..813999	+	81	Protein_799	hypothetical protein	-
EXA07_RS04055 (814120)	814120..814308	-	189	Protein_800	IS66 family transposase	-
EXA07_RS04060 (814369)	814369..815502	+	1134	WP_126125585.1	IS110-like element ISEc45 family transposase	-
EXA07_RS04070 (815755)	815755..816930	-	1176	Protein_802	IS66-like element ISEc23 family transposase	-
EXA07_RS04075 (816980)	816980..817719	+	740	Protein_803	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	811426..836053	24627

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14094.15 Da
• Isoelectric Point: 7.1881

>T118606 WP_000854762.1 NZ_CP035819:813296-813670 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T118606 NZ_CP035819:813296-813670 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT118606 WP_001295723.1 NZ_CP035819:812838-813206 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT118606 NZ_CP035819:812838-813206 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: S1NM52