Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 81609..82156 Replicon plasmid pE7476_162
Accession NZ_CP035818
Organism Escherichia coli strain E7476

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag EXA08_RS24880 Protein ID WP_001384452.1
Coordinates 81878..82156 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A2I2C2Y5
Locus tag EXA08_RS24875 Protein ID WP_000079948.1
Coordinates 81609..81878 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA08_RS24845 (77069) 77069..77635 + 567 WP_000469207.1 DUF2726 domain-containing protein -
EXA08_RS24850 (77632) 77632..78363 + 732 WP_001071488.1 type-F conjugative transfer system pilin acetylase TraX -
EXA08_RS24855 (78588) 78588..78770 - 183 WP_001393357.1 hypothetical protein -
EXA08_RS24860 (79277) 79277..79534 + 258 WP_000083823.1 replication regulatory protein RepA -
EXA08_RS25615 (79570) 79570..79806 - 237 WP_235588260.1 replication protein RepA -
EXA08_RS25620 (79797) 79797..79844 + 48 WP_235588261.1 hypothetical protein -
EXA08_RS24870 (79837) 79837..80694 + 858 WP_000130978.1 incFII family plasmid replication initiator RepA -
EXA08_RS24875 (81609) 81609..81878 + 270 WP_000079948.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
EXA08_RS24880 (81878) 81878..82156 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
EXA08_RS24885 (82202) 82202..83050 + 849 WP_001057994.1 exonuclease domain-containing protein -
EXA08_RS24890 (83167) 83167..83649 + 483 WP_032182006.1 hypothetical protein -
EXA08_RS24895 (84184) 84184..84786 - 603 WP_000517689.1 ProQ/FINO family protein -
EXA08_RS24900 (85140) 85140..85661 + 522 Protein_95 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - etpB / etpA / cfaA / csaC / csaE 1..162860 162860


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T118595 WP_001384452.1 NZ_CP035818:81878-82156 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T118595 NZ_CP035818:81878-82156 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10251.64 Da        Isoelectric Point: 10.1734

>AT118595 WP_000079948.1 NZ_CP035818:81609-81878 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLKRGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT118595 NZ_CP035818:81609-81878 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCAAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A2I2C2Y5

References