Detailed information of TA system TA118594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1272..1864
• Replicon: plasmid pE7476_162
• Accession: NZ_CP035818
• Organism: Escherichia coli strain E7476

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EXA08_RS24455
• Protein ID: WP_000513531.1
• Coordinates: 1272..1520 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EXA08_RS24460
• Protein ID: WP_000681610.1
• Coordinates: 1517..1864 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA08_RS24450 (496)	496..807	+	312	WP_000729511.1	hypothetical protein	-
EXA08_RS24455 (1272)	1272..1520	+	249	WP_000513531.1	type II toxin-antitoxin system HicA family toxin	Toxin
EXA08_RS24460 (1517)	1517..1864	+	348	WP_000681610.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EXA08_RS24465 (2091)	2091..3262	-	1172	Protein_4	IS91 family transposase	-
EXA08_RS24470 (3262)	3262..3627	-	366	WP_000124139.1	hypothetical protein	-
EXA08_RS24475 (3709)	3709..4311	+	603	Protein_6	DDE-type integrase/transposase/recombinase	-
EXA08_RS24480 (4513)	4513..6324	+	1812	WP_000260520.1	two-partner secretion system transporter EtpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	etpB / etpA / cfaA / csaC / csaE	1..162860	162860

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-80)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9298.86 Da
• Isoelectric Point: 9.9409

>T118594 WP_000513531.1 NZ_CP035818:1272-1520 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKDMQGSRVRFFNAEINHTILMHCPHPESYIKGGTLKTIKQNLNEAG
AL

Nucleotide

• Length: 249 bp

>T118594 NZ_CP035818:1272-1520 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTATT
TTCTTCTCTAGGATATGTCAAAAAAGACATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATTGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAACTATTAAACAGAACTTGAACGAGGCTGGG
GCTTTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13152.97 Da
• Isoelectric Point: 4.9966

>AT118594 WP_000681610.1 NZ_CP035818:1517-1864 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAKTLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVGNHRAGA

Nucleotide

• Length: 348 bp

>AT118594 NZ_CP035818:1517-1864 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAAAAACATTAGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGCCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCGCTGGAAAAAGA
AGTCGGTAATCATCGCGCAGGGGCTTAA