Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4667024..4667619 Replicon chromosome
Accession NZ_CP035817
Organism Escherichia coli strain E7476

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag EXA08_RS22905 Protein ID WP_000239579.1
Coordinates 4667024..4667374 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag EXA08_RS22910 Protein ID WP_001223208.1
Coordinates 4667368..4667619 (-) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA08_RS22885 (4662300) 4662300..4663322 - 1023 WP_001339490.1 ABC transporter permease -
EXA08_RS22890 (4663336) 4663336..4664838 - 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
EXA08_RS22895 (4665148) 4665148..4666104 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
EXA08_RS22900 (4666414) 4666414..4666944 + 531 WP_000055072.1 inorganic diphosphatase -
EXA08_RS22905 (4667024) 4667024..4667374 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
EXA08_RS22910 (4667368) 4667368..4667619 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
EXA08_RS22915 (4667832) 4667832..4668173 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
EXA08_RS22920 (4668176) 4668176..4671955 - 3780 WP_000060901.1 autotransporter assembly complex protein TamB -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(8-114)

Antitoxin

(6-49)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T118592 WP_000239579.1 NZ_CP035817:c4667374-4667024 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T118592 NZ_CP035817:c4667374-4667024 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT118592 WP_001223208.1 NZ_CP035817:c4667619-4667368 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT118592 NZ_CP035817:c4667619-4667368 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References