Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4497777..4498353 Replicon chromosome
Accession NZ_CP035817
Organism Escherichia coli strain E7476

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag EXA08_RS22055 Protein ID WP_001295743.1
Coordinates 4498066..4498353 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A6N8Q8R5
Locus tag EXA08_RS22050 Protein ID WP_001422870.1
Coordinates 4497777..4498079 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA08_RS22035 (4494373) 4494373..4496523 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
EXA08_RS22040 (4496573) 4496573..4496776 + 204 WP_000467859.1 YbdD/YjiX family protein -
EXA08_RS22045 (4496787) 4496787..4497743 + 957 WP_001295745.1 GTPase -
EXA08_RS22050 (4497777) 4497777..4498079 - 303 WP_001422870.1 BrnA antitoxin family protein Antitoxin
EXA08_RS22055 (4498066) 4498066..4498353 - 288 WP_001295743.1 BrnT family toxin Toxin
EXA08_RS22060 (4498550) 4498550..4499716 + 1167 WP_000800831.1 restriction endonuclease -
EXA08_RS22065 (4499780) 4499780..4501399 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
EXA08_RS22070 (4501389) 4501389..4502693 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T118590 WP_001295743.1 NZ_CP035817:c4498353-4498066 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T118590 NZ_CP035817:c4498353-4498066 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11582.09 Da        Isoelectric Point: 9.4646

>AT118590 WP_001422870.1 NZ_CP035817:c4498079-4497777 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRSGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT118590 NZ_CP035817:c4498079-4497777 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGATCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A6N8Q8R5

References