Detailed information of TA system TA118588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3939948..3940785
• Replicon: chromosome
• Accession: NZ_CP035817
• Organism: Escherichia coli strain E7476

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: EXA08_RS19360
• Protein ID: WP_000227784.1
• Coordinates: 3940243..3940785 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: EXA08_RS19355
• Protein ID: WP_001297137.1
• Coordinates: 3939948..3940259 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA08_RS19330 (3934968)	3934968..3935915	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
EXA08_RS19335 (3935937)	3935937..3937928	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
EXA08_RS19340 (3937918)	3937918..3938532	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
EXA08_RS19345 (3938532)	3938532..3938861	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
EXA08_RS19350 (3938873)	3938873..3939763	+	891	WP_000971336.1	heme o synthase	-
EXA08_RS19355 (3939948)	3939948..3940259	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
EXA08_RS19360 (3940243)	3940243..3940785	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
EXA08_RS19365 (3940841)	3940841..3941776	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
EXA08_RS19370 (3942184)	3942184..3943548	+	1365	WP_001000978.1	MFS transporter	-
EXA08_RS19375 (3943676)	3943676..3944167	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
EXA08_RS19380 (3944335)	3944335..3945246	+	912	WP_000705847.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T118588 WP_000227784.1 NZ_CP035817:3940243-3940785 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T118588 NZ_CP035817:3940243-3940785 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT118588 WP_001297137.1 NZ_CP035817:3939948-3940259 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT118588 NZ_CP035817:3939948-3940259 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3