Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3905871..3906489 Replicon chromosome
Accession NZ_CP035817
Organism Escherichia coli strain E7476

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EXA08_RS19190 Protein ID WP_001291435.1
Coordinates 3906271..3906489 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EXA08_RS19185 Protein ID WP_000344800.1
Coordinates 3905871..3906245 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA08_RS19175 (3900960) 3900960..3902153 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EXA08_RS19180 (3902176) 3902176..3905325 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
EXA08_RS19185 (3905871) 3905871..3906245 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EXA08_RS19190 (3906271) 3906271..3906489 + 219 WP_001291435.1 HHA domain-containing protein Toxin
EXA08_RS19195 (3906661) 3906661..3907212 + 552 WP_000102568.1 maltose O-acetyltransferase -
EXA08_RS19200 (3907328) 3907328..3907798 + 471 WP_000136192.1 YlaC family protein -
EXA08_RS19205 (3907962) 3907962..3909512 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EXA08_RS19210 (3909554) 3909554..3909907 - 354 WP_000878140.1 DUF1428 domain-containing protein -
EXA08_RS19220 (3910286) 3910286..3910597 + 312 WP_000409911.1 MGMT family protein -
EXA08_RS19225 (3910628) 3910628..3911200 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T118587 WP_001291435.1 NZ_CP035817:3906271-3906489 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T118587 NZ_CP035817:3906271-3906489 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT118587 WP_000344800.1 NZ_CP035817:3905871-3906245 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT118587 NZ_CP035817:3905871-3906245 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References