Detailed information of TA system TA118585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2858094..2858732
• Replicon: chromosome
• Accession: NZ_CP035817
• Organism: Escherichia coli strain E7476

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: EXA08_RS14025
• Protein ID: WP_000813794.1
• Coordinates: 2858556..2858732 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EXA08_RS14020
• Protein ID: WP_001270286.1
• Coordinates: 2858094..2858510 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA08_RS14000 (2853246)	2853246..2854187	-	942	WP_001251319.1	ABC transporter permease	-
EXA08_RS14005 (2854188)	2854188..2855201	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
EXA08_RS14010 (2855219)	2855219..2856364	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
EXA08_RS14015 (2856609)	2856609..2858015	-	1407	WP_001421323.1	PLP-dependent aminotransferase family protein	-
EXA08_RS14020 (2858094)	2858094..2858510	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
EXA08_RS14025 (2858556)	2858556..2858732	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
EXA08_RS14030 (2858954)	2858954..2859184	+	231	WP_000494244.1	YncJ family protein	-
EXA08_RS14035 (2859276)	2859276..2861237	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
EXA08_RS14040 (2861310)	2861310..2861846	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
EXA08_RS14045 (2861938)	2861938..2863113	+	1176	WP_001236319.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2863153..2864418	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T118585 WP_000813794.1 NZ_CP035817:c2858732-2858556 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T118585 NZ_CP035817:c2858732-2858556 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT118585 WP_001270286.1 NZ_CP035817:c2858510-2858094 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT118585 NZ_CP035817:c2858510-2858094 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB