Detailed information of TA system TA118573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1689355..1689980
• Replicon: chromosome
• Accession: NZ_CP035817
• Organism: Escherichia coli strain E7476

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EXA08_RS08235
• Protein ID: WP_000911330.1
• Coordinates: 1689582..1689980 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EXA08_RS08230
• Protein ID: WP_000450524.1
• Coordinates: 1689355..1689582 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA08_RS08205 (1685158)	1685158..1685628	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
EXA08_RS08210 (1685628)	1685628..1686200	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
EXA08_RS08215 (1686346)	1686346..1687224	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EXA08_RS08220 (1687241)	1687241..1688275	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
EXA08_RS08225 (1688488)	1688488..1689201	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EXA08_RS08230 (1689355)	1689355..1689582	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EXA08_RS08235 (1689582)	1689582..1689980	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
EXA08_RS08240 (1690127)	1690127..1690990	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
EXA08_RS08245 (1691005)	1691005..1693020	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
EXA08_RS08250 (1693094)	1693094..1693792	+	699	WP_000679823.1	esterase	-
EXA08_RS08255 (1693902)	1693902..1694102	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T118573 WP_000911330.1 NZ_CP035817:1689582-1689980 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T118573 NZ_CP035817:1689582-1689980 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT118573 WP_000450524.1 NZ_CP035817:1689355-1689582 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT118573 NZ_CP035817:1689355-1689582 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33