Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1183339..1183993 Replicon chromosome
Accession NZ_CP035817
Organism Escherichia coli strain E7476

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag EXA08_RS05725 Protein ID WP_000244772.1
Coordinates 1183586..1183993 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EXA08_RS05720 Protein ID WP_000354046.1
Coordinates 1183339..1183605 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA08_RS05695 (1178627) 1178627..1179370 + 744 WP_000951964.1 SDR family oxidoreductase -
EXA08_RS05700 (1179427) 1179427..1180860 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
EXA08_RS05705 (1180905) 1180905..1181216 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
EXA08_RS05710 (1181380) 1181380..1182039 + 660 WP_000250274.1 hemolysin III family protein -
EXA08_RS05715 (1182116) 1182116..1183096 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EXA08_RS05720 (1183339) 1183339..1183605 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EXA08_RS05725 (1183586) 1183586..1183993 + 408 WP_000244772.1 protein YgfX Toxin
EXA08_RS05730 (1184033) 1184033..1184554 - 522 WP_001055874.1 flavodoxin FldB -
EXA08_RS05735 (1184666) 1184666..1185562 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EXA08_RS05740 (1185587) 1185587..1186297 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EXA08_RS05745 (1186303) 1186303..1188036 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T118571 WP_000244772.1 NZ_CP035817:1183586-1183993 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118571 NZ_CP035817:1183586-1183993 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT118571 WP_000354046.1 NZ_CP035817:1183339-1183605 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118571 NZ_CP035817:1183339-1183605 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References