Detailed information of TA system TA118567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 16305..16852
• Replicon: plasmid pB7A_66
• Accession: NZ_CP035815
• Organism: Escherichia coli strain B7A

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G7QEJ9
• Locus tag: EXA09_RS25335
• Protein ID: WP_001384452.1
• Coordinates: 16305..16583 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: K4JYR0
• Locus tag: EXA09_RS25340
• Protein ID: WP_000079928.1
• Coordinates: 16583..16852 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA09_RS25295 (11647)	11647..11949	+	303	WP_235837087.1	IS66 family insertion sequence element accessory protein TnpB	-
EXA09_RS25300 (11946)	11946..12197	+	252	Protein_19	IS66 family insertion sequence element accessory protein TnpB	-
EXA09_RS26150 (12192)	12192..12346	+	155	Protein_20	transposase	-
EXA09_RS25310 (12380)	12380..12556	-	177	Protein_21	transposase domain-containing protein	-
EXA09_RS25315 (12785)	12785..13567	-	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
EXA09_RS25320 (13564)	13564..14586	-	1023	WP_001297099.1	IS21-like element IS100 family transposase	-
EXA09_RS25325 (14812)	14812..15294	-	483	WP_001311056.1	hypothetical protein	-
EXA09_RS25330 (15411)	15411..16259	-	849	WP_042815954.1	3'-5' exonuclease	-
EXA09_RS25335 (16305)	16305..16583	-	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	Toxin
EXA09_RS25340 (16583)	16583..16852	-	270	WP_000079928.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
EXA09_RS25345 (17768)	17768..18625	-	858	WP_000131014.1	incFII family plasmid replication initiator RepA	-
EXA09_RS25350 (18618)	18618..18692	-	75	WP_001365705.1	RepA leader peptide Tap	-
EXA09_RS25355 (18928)	18928..19182	-	255	WP_000083830.1	replication regulatory protein RepA	-
EXA09_RS25360 (19422)	19422..20012	-	591	WP_001297502.1	DUF2726 domain-containing protein	-
EXA09_RS25365 (20164)	20164..20790	-	627	WP_000794191.1	NYN domain-containing protein	-
EXA09_RS25370 (21202)	21202..21411	-	210	WP_001297500.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa / eltA / eltB	1..66338	66338

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.38 Da
• Isoelectric Point: 8.4615

>T118567 WP_001384452.1 NZ_CP035815:c16583-16305 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T118567 NZ_CP035815:c16583-16305 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
TGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10175.50 Da
• Isoelectric Point: 7.3210

>AT118567 WP_000079928.1 NZ_CP035815:c16852-16583 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT118567 NZ_CP035815:c16852-16583 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A829CL36

Antitoxin

• AlphaFold DB: A0A5W2J4D0