Detailed information of TA system TA118566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 91334..91893
• Replicon: plasmid pB7A_99
• Accession: NZ_CP035813
• Organism: Escherichia coli strain B7A

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EXA09_RS24735
• Protein ID: WP_000421261.1
• Coordinates: 91334..91609 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EXA09_RS24740
• Protein ID: WP_001297026.1
• Coordinates: 91609..91893 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA09_RS24700	87357..87611	+	255	WP_000343106.1	hypothetical protein	-
EXA09_RS24705	87611..88201	+	591	WP_001401589.1	hypothetical protein	-
EXA09_RS24710	88414..89466	+	1053	WP_000655519.1	hypothetical protein	-
EXA09_RS24715	89642..90244	+	603	WP_000521606.1	ProQ/FINO family protein	-
EXA09_RS24720	90272..90490	+	219	Protein_107	hypothetical protein	-
EXA09_RS24725	90490..90756	+	267	WP_000627832.1	hypothetical protein	-
EXA09_RS24730	91025..91228	-	204	Protein_109	hypothetical protein	-
EXA09_RS24735	91334..91609	-	276	WP_000421261.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EXA09_RS24740	91609..91893	-	285	WP_001297026.1	ribbon-helix-helix domain-containing protein	Antitoxin
EXA09_RS24745	92801..93832	-	1032	WP_001297009.1	plasmid replication initiator RepA	-
EXA09_RS24750	94533..95174	+	642	WP_000335851.1	transcription termination/antitermination NusG family protein	-
EXA09_RS24755	95315..95980	+	666	WP_000154140.1	hypothetical protein	-
EXA09_RS26135	96335..96481	+	147	WP_235777688.1	PilI type IV pilus biogenesis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA1 / sul1 / qacE / aadA2 / tet(C)	-	1..99955	99955

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T118566 WP_000421261.1 NZ_CP035813:c91609-91334 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEVRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T118566 NZ_CP035813:c91609-91334 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAGTCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10502.95 Da
• Isoelectric Point: 5.6558

>AT118566 WP_001297026.1 NZ_CP035813:c91893-91609 [Escherichia coli]
MQMKNNTVQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT118566 NZ_CP035813:c91893-91609 [Escherichia coli]
ATGCAAATGAAAAACAATACCGTACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTCATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA