Detailed information of TA system TA118565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4854167..4855004
• Replicon: chromosome
• Accession: NZ_CP035812
• Organism: Escherichia coli strain B7A

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: EXA09_RS23755
• Protein ID: WP_000227784.1
• Coordinates: 4854462..4855004 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: EXA09_RS23750
• Protein ID: WP_001297137.1
• Coordinates: 4854167..4854478 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA09_RS23725 (4849187)	4849187..4850134	+	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-
EXA09_RS23730 (4850156)	4850156..4852147	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
EXA09_RS23735 (4852137)	4852137..4852751	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
EXA09_RS23740 (4852751)	4852751..4853080	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
EXA09_RS23745 (4853092)	4853092..4853982	+	891	WP_000971336.1	heme o synthase	-
EXA09_RS23750 (4854167)	4854167..4854478	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
EXA09_RS23755 (4854462)	4854462..4855004	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
EXA09_RS23760 (4855060)	4855060..4855995	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
EXA09_RS23765 (4856403)	4856403..4857767	+	1365	WP_001000978.1	MFS transporter	-
EXA09_RS23770 (4857895)	4857895..4858386	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
EXA09_RS23775 (4858554)	4858554..4859465	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T118565 WP_000227784.1 NZ_CP035812:4854462-4855004 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T118565 NZ_CP035812:4854462-4855004 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT118565 WP_001297137.1 NZ_CP035812:4854167-4854478 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT118565 NZ_CP035812:4854167-4854478 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3