Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4781884..4782502 Replicon chromosome
Accession NZ_CP035812
Organism Escherichia coli strain B7A

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EXA09_RS23310 Protein ID WP_001291435.1
Coordinates 4782284..4782502 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EXA09_RS23305 Protein ID WP_000344800.1
Coordinates 4781884..4782258 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA09_RS23295 (4776973) 4776973..4778166 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EXA09_RS23300 (4778189) 4778189..4781338 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
EXA09_RS23305 (4781884) 4781884..4782258 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EXA09_RS23310 (4782284) 4782284..4782502 + 219 WP_001291435.1 HHA domain-containing protein Toxin
EXA09_RS23315 (4782674) 4782674..4783225 + 552 WP_000102564.1 maltose O-acetyltransferase -
EXA09_RS23320 (4783341) 4783341..4783811 + 471 WP_000136192.1 YlaC family protein -
EXA09_RS23325 (4783975) 4783975..4785525 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EXA09_RS23330 (4785567) 4785567..4785920 - 354 WP_000878141.1 DUF1428 family protein -
EXA09_RS23340 (4786299) 4786299..4786610 + 312 WP_000409911.1 MGMT family protein -
EXA09_RS23345 (4786641) 4786641..4787213 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T118564 WP_001291435.1 NZ_CP035812:4782284-4782502 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T118564 NZ_CP035812:4782284-4782502 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT118564 WP_000344800.1 NZ_CP035812:4781884-4782258 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT118564 NZ_CP035812:4781884-4782258 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References