Detailed information of TA system TA118553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3599003..3599641
• Replicon: chromosome
• Accession: NZ_CP035812
• Organism: Escherichia coli strain B7A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: EXA09_RS17555
• Protein ID: WP_000813794.1
• Coordinates: 3599003..3599179 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EXA09_RS17560
• Protein ID: WP_001270286.1
• Coordinates: 3599225..3599641 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA09_RS17535 (3594622)	3594622..3595797	-	1176	WP_001236258.1	BenE family transporter YdcO	-
EXA09_RS17540 (3595889)	3595889..3596425	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
EXA09_RS17545 (3596498)	3596498..3598459	+	1962	WP_042815132.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
EXA09_RS17550 (3598551)	3598551..3598781	-	231	WP_000494244.1	YncJ family protein	-
EXA09_RS17555 (3599003)	3599003..3599179	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
EXA09_RS17560 (3599225)	3599225..3599641	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
EXA09_RS17565 (3599720)	3599720..3601126	+	1407	WP_001296748.1	PLP-dependent aminotransferase family protein	-
EXA09_RS17570 (3601371)	3601371..3602516	+	1146	WP_001296759.1	ABC transporter substrate-binding protein	-
EXA09_RS17575 (3602534)	3602534..3603547	+	1014	WP_000220416.1	ABC transporter ATP-binding protein	-
EXA09_RS17580 (3603548)	3603548..3604489	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3593317..3594582	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T118553 WP_000813794.1 NZ_CP035812:3599003-3599179 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T118553 NZ_CP035812:3599003-3599179 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT118553 WP_001270286.1 NZ_CP035812:3599225-3599641 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT118553 NZ_CP035812:3599225-3599641 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB