Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2498617..2499242 Replicon chromosome
Accession NZ_CP035812
Organism Escherichia coli strain B7A

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag EXA09_RS11870 Protein ID WP_000911329.1
Coordinates 2498844..2499242 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag EXA09_RS11865 Protein ID WP_000450524.1
Coordinates 2498617..2498844 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA09_RS11840 (2494419) 2494419..2494889 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
EXA09_RS11845 (2494889) 2494889..2495461 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
EXA09_RS11850 (2495607) 2495607..2496485 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
EXA09_RS11855 (2496502) 2496502..2497536 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
EXA09_RS11860 (2497749) 2497749..2498462 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
EXA09_RS11865 (2498617) 2498617..2498844 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
EXA09_RS11870 (2498844) 2498844..2499242 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
EXA09_RS11875 (2499389) 2499389..2500252 + 864 WP_001267507.1 neutral zinc metallopeptidase -
EXA09_RS11880 (2500267) 2500267..2502282 + 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
EXA09_RS11885 (2502356) 2502356..2503054 + 699 WP_000679812.1 esterase -
EXA09_RS11890 (2503164) 2503164..2503364 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2394672..2559799 165127


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T118552 WP_000911329.1 NZ_CP035812:2498844-2499242 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T118552 NZ_CP035812:2498844-2499242 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT118552 WP_000450524.1 NZ_CP035812:2498617-2498844 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT118552 NZ_CP035812:2498617-2498844 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References