Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2033640..2034294 Replicon chromosome
Accession NZ_CP035812
Organism Escherichia coli strain B7A

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag EXA09_RS09635 Protein ID WP_000244772.1
Coordinates 2033887..2034294 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EXA09_RS09630 Protein ID WP_000354046.1
Coordinates 2033640..2033906 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA09_RS09605 (2028928) 2028928..2029671 + 744 WP_000951940.1 SDR family oxidoreductase -
EXA09_RS09610 (2029728) 2029728..2031161 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
EXA09_RS09615 (2031206) 2031206..2031517 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
EXA09_RS09620 (2031681) 2031681..2032340 + 660 WP_000250274.1 hemolysin III family protein -
EXA09_RS09625 (2032417) 2032417..2033397 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EXA09_RS09630 (2033640) 2033640..2033906 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EXA09_RS09635 (2033887) 2033887..2034294 + 408 WP_000244772.1 protein YgfX Toxin
EXA09_RS09640 (2034334) 2034334..2034855 - 522 WP_001055874.1 flavodoxin FldB -
EXA09_RS09645 (2034967) 2034967..2035863 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EXA09_RS09650 (2035888) 2035888..2036598 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EXA09_RS09655 (2036604) 2036604..2038337 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T118550 WP_000244772.1 NZ_CP035812:2033887-2034294 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118550 NZ_CP035812:2033887-2034294 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT118550 WP_000354046.1 NZ_CP035812:2033640-2033906 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118550 NZ_CP035812:2033640-2033906 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References