Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 660419..660916 Replicon chromosome
Accession NZ_CP035812
Organism Escherichia coli strain B7A

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EXA09_RS03065 Protein ID WP_001173343.1
Coordinates 660419..660592 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EXA09_RS03070 Protein ID WP_000398619.1
Coordinates 660620..660916 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA09_RS03055 (657089) 657089..658546 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
EXA09_RS03060 (658783) 658783..660300 + 1518 WP_001295063.1 lysine--tRNA ligase -
EXA09_RS03065 (660419) 660419..660592 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EXA09_RS03070 (660620) 660620..660916 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EXA09_RS03075 (661143) 661143..661415 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
EXA09_RS03080 (661427) 661427..661657 - 231 WP_000371699.1 4Fe-4S mono-cluster protein YjdI -
EXA09_RS03085 (661838) 661838..663469 + 1632 WP_001216460.1 sensor histidine kinase -
EXA09_RS03090 (663466) 663466..664185 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T118546 WP_001173343.1 NZ_CP035812:c660592-660419 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T118546 NZ_CP035812:c660592-660419 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT118546 WP_000398619.1 NZ_CP035812:c660916-660620 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT118546 NZ_CP035812:c660916-660620 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References