Detailed information of TA system TA118545

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 460320..461152
• Replicon: chromosome
• Accession: NZ_CP035812
• Organism: Escherichia coli strain B7A

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: EXA09_RS02130
• Protein ID: WP_000854753.1
• Coordinates: 460320..460694 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: EXA09_RS02135
• Protein ID: WP_021542768.1
• Coordinates: 460784..461152 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA09_RS02100 (455370)	455370..456350	+	981	WP_001295601.1	sialate O-acetylesterase	-
EXA09_RS02105 (456358)	456358..457008	-	651	WP_001037966.1	HNH endonuclease	-
EXA09_RS25945 (457145)	457145..457288	+	144	Protein_412	HNH endonuclease	-
EXA09_RS02110 (458056)	458056..458205	-	150	Protein_413	restriction endonuclease subunit M	-
EXA09_RS02115 (458290)	458290..458487	-	198	WP_000839281.1	DUF957 domain-containing protein	-
EXA09_RS25950 (458499)	458499..458603	-	105	Protein_415	DUF5983 family protein	-
EXA09_RS02125 (459937)	459937..460323	-	387	Protein_417	DUF5983 family protein	-
EXA09_RS02130 (460320)	460320..460694	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
EXA09_RS02135 (460784)	460784..461152	-	369	WP_021542768.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EXA09_RS02140 (461315)	461315..461536	-	222	WP_000692345.1	DUF987 domain-containing protein	-
EXA09_RS02145 (461599)	461599..462075	-	477	WP_001297237.1	RadC family protein	-
EXA09_RS02150 (462091)	462091..462576	-	486	WP_000849588.1	antirestriction protein	-
EXA09_RS02155 (462631)	462631..463449	-	819	WP_001234620.1	DUF932 domain-containing protein	-
EXA09_RS02160 (463549)	463549..463782	-	234	WP_001119729.1	DUF905 family protein	-
EXA09_RS02165 (463861)	463861..464316	-	456	WP_000581504.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T118545 WP_000854753.1 NZ_CP035812:c460694-460320 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T118545 NZ_CP035812:c460694-460320 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13573.33 Da
• Isoelectric Point: 6.4789

>AT118545 WP_021542768.1 NZ_CP035812:c461152-460784 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWRLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT118545 NZ_CP035812:c461152-460784 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGAGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0