Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 422743..423319 Replicon chromosome
Accession NZ_CP035812
Organism Escherichia coli strain B7A

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag EXA09_RS01935 Protein ID WP_001297643.1
Coordinates 423032..423319 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag EXA09_RS01930 Protein ID WP_000063156.1
Coordinates 422743..423045 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA09_RS01915 (419339) 419339..421489 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
EXA09_RS01920 (421539) 421539..421742 + 204 WP_000467859.1 YbdD/YjiX family protein -
EXA09_RS01925 (421753) 421753..422709 + 957 WP_001297640.1 GTPase -
EXA09_RS01930 (422743) 422743..423045 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
EXA09_RS01935 (423032) 423032..423319 - 288 WP_001297643.1 BrnT family toxin Toxin
EXA09_RS01940 (423518) 423518..424215 - 698 Protein_378 IS1 family transposase -
EXA09_RS01945 (424272) 424272..424367 + 96 Protein_379 dienelactone hydrolase family protein -
EXA09_RS01950 (424513) 424513..425745 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
EXA09_RS01955 (425786) 425786..427066 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 423518..423727 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T118544 WP_001297643.1 NZ_CP035812:c423319-423032 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T118544 NZ_CP035812:c423319-423032 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT118544 WP_000063156.1 NZ_CP035812:c423045-422743 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT118544 NZ_CP035812:c423045-422743 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References