Detailed information of TA system TA118537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 1676078..1676585
• Replicon: chromosome
• Accession: NZ_CP035807
• Organism: Thiospirochaeta perfilievii strain P

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: EW093_RS07580
• Protein ID: WP_149567812.1
• Coordinates: 1676313..1676585 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: EW093_RS07575
• Protein ID: WP_149567811.1
• Coordinates: 1676078..1676326 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW093_RS17300	1672155..1672325	-	171	WP_187759877.1	hypothetical protein	-
EW093_RS07565	1672551..1673063	-	513	WP_149567809.1	hypothetical protein	-
EW093_RS07570	1673449..1674693	-	1245	WP_149567810.1	hypothetical protein	-
EW093_RS07575	1676078..1676326	-	249	WP_149567811.1	BrnA antitoxin family protein	Antitoxin
EW093_RS07580	1676313..1676585	-	273	WP_149567812.1	BrnT family toxin	Toxin
EW093_RS07585	1677427..1678746	-	1320	WP_149567813.1	AAA family ATPase	-
EW093_RS07590	1679435..1680085	+	651	WP_149567814.1	redox-sensing transcriptional repressor Rex	-
EW093_RS07595	1680137..1681213	+	1077	WP_149567815.1	3-phosphoserine/phosphohydroxythreonine transaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	1670314..1678746	8432

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (23-77)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10886.15 Da
• Isoelectric Point: 6.4705

>T118537 WP_149567812.1 NZ_CP035807:c1676585-1676313 [Thiospirochaeta perfilievii]
MKFEWDENKNQSNYEKHGIYFDEAVEVFKSDCLTWIDDRKNYGETREITIGEVSKNIVLVLVHTDRFGNIRIISARKANE
RERSKYYDYR

Nucleotide

• Length: 273 bp

>T118537 NZ_CP035807:c1676585-1676313 [Thiospirochaeta perfilievii]
ATGAAGTTTGAATGGGATGAAAATAAAAATCAATCTAATTATGAGAAACATGGTATTTATTTTGATGAAGCCGTAGAGGT
TTTTAAGAGTGATTGTTTAACTTGGATTGATGATAGAAAAAATTATGGAGAAACCAGAGAAATAACAATTGGAGAAGTAA
GTAAAAATATTGTTTTAGTACTTGTTCACACTGACAGGTTTGGAAATATAAGAATTATTTCAGCTAGGAAAGCTAATGAA
AGAGAGAGGAGTAAATATTATGACTATCGATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9694.09 Da
• Isoelectric Point: 9.6245

>AT118537 WP_149567811.1 NZ_CP035807:c1676326-1676078 [Thiospirochaeta perfilievii]
MTIDKKRLHEIKSIKDKDIDYSDIPELDKTFWANVKPVYPKQKKAISIRLDQDIIDYFKSDGRGYQSKINNILRSYVEAH
HA

Nucleotide

• Length: 249 bp

>AT118537 NZ_CP035807:c1676326-1676078 [Thiospirochaeta perfilievii]
ATGACTATCGATAAAAAAAGATTACATGAAATAAAATCAATAAAAGATAAAGATATTGATTATTCAGATATACCTGAATT
AGATAAAACATTCTGGGCTAATGTAAAACCTGTGTACCCAAAGCAAAAGAAAGCAATAAGCATAAGATTAGATCAAGATA
TAATAGATTATTTCAAATCGGATGGTAGAGGGTATCAATCTAAAATAAATAATATTTTAAGAAGTTATGTTGAAGCACAT
CATGCATGA