Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 243948..244507 Replicon chromosome
Accession NZ_CP035795
Organism Brucella melitensis strain B15

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag EXD78_RS01350 Protein ID WP_002966807.1
Coordinates 244262..244507 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID C0RIV1
Locus tag EXD78_RS01345 Protein ID WP_004683740.1
Coordinates 243948..244262 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXD78_RS01295 239653..239856 - 204 WP_004683737.1 hypothetical protein -
EXD78_RS01300 239859..240074 - 216 WP_005968947.1 hypothetical protein -
EXD78_RS01305 240071..240274 - 204 WP_002964088.1 hypothetical protein -
EXD78_RS01310 240324..241028 + 705 WP_005968944.1 hypothetical protein -
EXD78_RS01315 241025..241255 + 231 WP_002964091.1 hypothetical protein -
EXD78_RS01320 241252..241527 + 276 WP_002964092.1 hypothetical protein -
EXD78_RS01325 241550..242026 + 477 WP_002966806.1 hypothetical protein -
EXD78_RS01330 242372..243082 + 711 WP_004683739.1 porin family protein -
EXD78_RS01335 243430..243600 - 171 WP_002964095.1 DUF1508 domain-containing protein -
EXD78_RS01345 243948..244262 - 315 WP_004683740.1 BrnA antitoxin family protein Antitoxin
EXD78_RS01350 244262..244507 - 246 WP_002966807.1 BrnT family toxin Toxin
EXD78_RS01355 244640..245278 + 639 Protein_239 tRNA-guanine transglycosylase -
EXD78_RS01360 245348..246106 + 759 Protein_240 IS5 family transposase -
EXD78_RS01365 246107..246799 + 693 WP_006135935.1 hypothetical protein -
EXD78_RS01370 247211..248662 + 1452 WP_006135932.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - wboA / wbdA 237881..251978 14097
- flank IS/Tn - - 245348..245716 368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T118528 WP_002966807.1 NZ_CP035795:c244507-244262 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T118528 NZ_CP035795:c244507-244262 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11653.48 Da        Isoelectric Point: 10.5824

>AT118528 WP_004683740.1 NZ_CP035795:c244262-243948 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT118528 NZ_CP035795:c244262-243948 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure
AlphaFold DB C0RIV1

References