Detailed information of TA system TA118527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 243949..244508
• Replicon: chromosome
• Accession: NZ_CP035793
• Organism: Brucella melitensis strain B29

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7U8PD14
• Locus tag: EXD77_RS01350
• Protein ID: WP_002966807.1
• Coordinates: 244263..244508 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: EXD77_RS01345
• Protein ID: WP_004683740.1
• Coordinates: 243949..244263 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXD77_RS01295	239654..239857	-	204	WP_004683737.1	hypothetical protein	-
EXD77_RS01300	239860..240075	-	216	WP_005968947.1	hypothetical protein	-
EXD77_RS01305	240072..240275	-	204	WP_002964088.1	hypothetical protein	-
EXD77_RS01310	240325..241029	+	705	WP_005968944.1	hypothetical protein	-
EXD77_RS01315	241026..241256	+	231	WP_002964091.1	hypothetical protein	-
EXD77_RS01320	241253..241528	+	276	WP_002964092.1	hypothetical protein	-
EXD77_RS01325	241551..242027	+	477	WP_002966806.1	hypothetical protein	-
EXD77_RS01330	242373..243083	+	711	WP_004683739.1	porin family protein	-
EXD77_RS01335	243431..243601	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
EXD77_RS17000	243600..243833	+	234	WP_002969999.1	hypothetical protein	-
EXD77_RS01345	243949..244263	-	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
EXD77_RS01350	244263..244508	-	246	WP_002966807.1	BrnT family toxin	Toxin
EXD77_RS01355	244641..245279	+	639	Protein_240	tRNA-guanine transglycosylase	-
EXD77_RS01360	245349..246107	+	759	Protein_241	IS5 family transposase	-
EXD77_RS01365	246108..246800	+	693	WP_006135935.1	hypothetical protein	-
EXD77_RS01370	247212..248663	+	1452	WP_006135932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	237882..251979	14097
	-	flank	IS/Tn	-	-	245349..245717	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9162.82 Da
• Isoelectric Point: 10.3158

>T118527 WP_002966807.1 NZ_CP035793:c244508-244263 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T118527 NZ_CP035793:c244508-244263 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT118527 WP_004683740.1 NZ_CP035793:c244263-243949 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT118527 NZ_CP035793:c244263-243949 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U8PD14

Antitoxin

• AlphaFold DB: C0RIV1