Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 77842..78485 Replicon plasmid pRM8843-C1
Accession NZ_CP035773
Organism Escherichia coli O145 strain RM8843-C1

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag EW648_RS27180 Protein ID WP_001044768.1
Coordinates 77842..78258 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag EW648_RS27185 Protein ID WP_001261287.1
Coordinates 78255..78485 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW648_RS27155 74387..74734 - 348 WP_000631725.1 IS66 family insertion sequence element accessory protein TnpB -
EW648_RS27160 74731..75405 - 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -
EW648_RS27165 75459..75713 - 255 WP_097455692.1 tyrosine-type recombinase/integrase -
EW648_RS27175 77135..77680 + 546 WP_001165114.1 hypothetical protein -
EW648_RS27180 77842..78258 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
EW648_RS27185 78255..78485 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EW648_RS27190 79045..79458 + 414 WP_000465041.1 hypothetical protein -
EW648_RS27195 79460..80242 + 783 WP_001164205.1 site-specific integrase -
EW648_RS27200 80415..80768 + 354 WP_000864810.1 colicin M immunity protein -
EW648_RS27205 80776..80967 - 192 Protein_82 lipid II-degrading bacteriocin -
EW648_RS27210 80971..81114 - 144 Protein_83 transcriptional regulator -
EW648_RS27215 81181..82620 - 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - espP / toxB / hlyD / hlyB / hlyA / hlyC 1..88752 88752


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T118509 WP_001044768.1 NZ_CP035773:c78258-77842 [Escherichia coli O145]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T118509 NZ_CP035773:c78258-77842 [Escherichia coli O145]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT118509 WP_001261287.1 NZ_CP035773:c78485-78255 [Escherichia coli O145]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT118509 NZ_CP035773:c78485-78255 [Escherichia coli O145]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References