Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4261454..4262085 Replicon chromosome
Accession NZ_CP035772
Organism Escherichia coli O145 strain RM8843-C1

Toxin (Protein)


Gene name hicA Uniprot ID A0A7U9LJQ2
Locus tag EW648_RS21265 Protein ID WP_001259387.1
Coordinates 4261810..4262085 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag EW648_RS21260 Protein ID WP_000593555.1
Coordinates 4261454..4261813 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW648_RS21230 4256566..4257039 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
EW648_RS21235 4257036..4257317 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
EW648_RS21240 4257394..4258752 + 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
EW648_RS21245 4258745..4260253 + 1509 WP_001400448.1 carbohydrate kinase -
EW648_RS21250 4260243..4260512 + 270 WP_000933061.1 HPr family phosphocarrier protein -
EW648_RS21255 4260544..4261404 + 861 WP_001131788.1 class II aldolase -
EW648_RS21260 4261454..4261813 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EW648_RS21265 4261810..4262085 - 276 WP_001259387.1 type II toxin-antitoxin system HicA family toxin Toxin
EW648_RS21270 4262158..4263282 - 1125 WP_001314210.1 ABC transporter permease -
EW648_RS21275 4263282..4266017 - 2736 WP_000149086.1 ribosome-associated ATPase/putative transporter RbbA -
EW648_RS21280 4266014..4267081 - 1068 WP_000361517.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10214.91 Da        Isoelectric Point: 10.7076

>T118505 WP_001259387.1 NZ_CP035772:c4262085-4261810 [Escherichia coli O145]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESQVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T118505 NZ_CP035772:c4262085-4261810 [Escherichia coli O145]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACAGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT118505 WP_000593555.1 NZ_CP035772:c4261813-4261454 [Escherichia coli O145]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT118505 NZ_CP035772:c4261813-4261454 [Escherichia coli O145]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAGCTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LJQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References