Detailed information of TA system TA118488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2447911..2448681
• Replicon: chromosome
• Accession: NZ_CP035772
• Organism: Escherichia coli O145 strain RM8843-C1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: EW648_RS12250
• Protein ID: WP_001401034.1
• Coordinates: 2448262..2448681 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: EW648_RS12245
• Protein ID: WP_001401035.1
• Coordinates: 2447911..2448177 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW648_RS12245	2447911..2448177	+	267	WP_001401035.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
EW648_RS12250	2448262..2448681	+	420	WP_001401034.1	HipA domain-containing protein	Toxin
EW648_RS12255	2448859..2449145	+	287	Protein_2399	helix-turn-helix transcriptional regulator	-
EW648_RS12260	2449579..2450142	+	564	WP_000768279.1	type 1 fimbrial major subunit FimA	-
EW648_RS12265	2450503..2451213	+	711	WP_001195165.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	2442523..2463129	20606

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (35-130)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15706.14 Da
• Isoelectric Point: 5.2713

>T118488 WP_001401034.1 NZ_CP035772:2448262-2448681 [Escherichia coli O145]
MAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTALLRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATL
DLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRWNTTRFSLGFCWCA

Nucleotide

• Length: 420 bp

>T118488 NZ_CP035772:2448262-2448681 [Escherichia coli O145]
ATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAGCAGATATCCCGCTAGGCATGAT
TAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCGCTGCTCAGAATAGGCAATGACTGGT
GCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGGGAAATCAGGCAGCCCAATGCGACGCTC
GATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTGGGTTGAATGTTCCGGACGCAGA
AATCATTAAAGCGGGAAATGTGCGTGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGGAATACTACTAGATTCTCCCTTG
GTTTCTGTTGGTGCGCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10077.48 Da
• Isoelectric Point: 4.9632

>AT118488 WP_001401035.1 NZ_CP035772:2447911-2448177 [Escherichia coli O145]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT118488 NZ_CP035772:2447911-2448177 [Escherichia coli O145]
ATGATGAGCTTTCAGAAGATCTATAGCCCAATGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCACTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA