Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 483501..484119 Replicon chromosome
Accession NZ_CP035772
Organism Escherichia coli O145 strain RM8843-C1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EW648_RS02230 Protein ID WP_001291435.1
Coordinates 483501..483719 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EW648_RS02235 Protein ID WP_000344800.1
Coordinates 483745..484119 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW648_RS02195 478790..479362 + 573 WP_000779831.1 YbaY family lipoprotein -
EW648_RS02200 479393..479704 - 312 WP_000409911.1 MGMT family protein -
EW648_RS02210 480083..480436 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EW648_RS02215 480478..482028 - 1551 WP_024221674.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EW648_RS02220 482192..482662 - 471 WP_000136192.1 YlaC family protein -
EW648_RS02225 482778..483329 - 552 WP_000102564.1 maltose O-acetyltransferase -
EW648_RS02230 483501..483719 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
EW648_RS02235 483745..484119 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EW648_RS02240 484665..487814 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
EW648_RS02245 487837..489030 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T118481 WP_001291435.1 NZ_CP035772:c483719-483501 [Escherichia coli O145]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T118481 NZ_CP035772:c483719-483501 [Escherichia coli O145]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT118481 WP_000344800.1 NZ_CP035772:c484119-483745 [Escherichia coli O145]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT118481 NZ_CP035772:c484119-483745 [Escherichia coli O145]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References