Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
Location | 54755..55305 | Replicon | chromosome |
Accession | NZ_CP035772 | ||
Organism | Escherichia coli O145 strain RM8843-C1 |
Toxin (Protein)
Gene name | ccdB | Uniprot ID | Q32K46 |
Locus tag | EW648_RS00250 | Protein ID | WP_001160974.1 |
Coordinates | 54991..55305 (+) | Length | 105 a.a. |
Antitoxin (Protein)
Gene name | ccdA | Uniprot ID | V0VEX8 |
Locus tag | EW648_RS00245 | Protein ID | WP_000125566.1 |
Coordinates | 54755..54988 (+) | Length | 78 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
EW648_RS00220 | 49826..50113 | + | 288 | WP_000203748.1 | ferredoxin-like protein FixX | - |
EW648_RS00225 | 50171..51502 | + | 1332 | WP_001183192.1 | MFS transporter | - |
EW648_RS00230 | 51613..52143 | + | 531 | WP_000600725.1 | glutathione-regulated potassium-efflux system oxidoreductase KefF | - |
EW648_RS00235 | 52136..53998 | + | 1863 | WP_000377137.1 | glutathione-regulated potassium-efflux system protein KefC | - |
EW648_RS00240 | 54190..54669 | + | 480 | WP_024221573.1 | type 3 dihydrofolate reductase | - |
EW648_RS00245 | 54755..54988 | + | 234 | WP_000125566.1 | type II toxin-antitoxin system CcdA family antitoxin | Antitoxin |
EW648_RS00250 | 54991..55305 | + | 315 | WP_001160974.1 | CcdB family protein | Toxin |
EW648_RS00255 | 55302..56150 | - | 849 | WP_000257203.1 | bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH | - |
EW648_RS00260 | 56157..56534 | - | 378 | WP_000610901.1 | Co2+/Mg2+ efflux protein ApaG | - |
EW648_RS00265 | 56537..57358 | - | 822 | WP_001065381.1 | 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA | - |
EW648_RS00270 | 57355..58344 | - | 990 | WP_000241240.1 | 4-hydroxythreonine-4-phosphate dehydrogenase PdxA | - |
EW648_RS00275 | 58344..59630 | - | 1287 | WP_000800457.1 | peptidylprolyl isomerase SurA | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 105 a.a. Molecular weight: 11737.84 Da Isoelectric Point: 9.8517
>T118480 WP_001160974.1 NZ_CP035772:54991-55305 [Escherichia coli O145]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI
Download Length: 315 bp
>T118480 NZ_CP035772:54991-55305 [Escherichia coli O145]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA
Antitoxin
Download Length: 78 a.a. Molecular weight: 8530.53 Da Isoelectric Point: 5.8062
>AT118480 WP_000125566.1 NZ_CP035772:54755-54988 [Escherichia coli O145]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF
Download Length: 234 bp
>AT118480 NZ_CP035772:54755-54988 [Escherichia coli O145]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A7U9LRR3 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A7U9LTM9 |