Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4218238..4219038 Replicon chromosome
Accession NZ_CP035770
Organism Escherichia coli O145 strain RM8988-C1

Toxin (Protein)


Gene name ataT Uniprot ID U9Y257
Locus tag EW646_RS21015 Protein ID WP_000342447.1
Coordinates 4218238..4218765 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID U9Y285
Locus tag EW646_RS21020 Protein ID WP_001277109.1
Coordinates 4218772..4219038 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW646_RS20990 4213312..4214079 - 768 WP_000082096.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
EW646_RS20995 4214076..4215353 - 1278 WP_000803782.1 branched chain amino acid ABC transporter permease LivM -
EW646_RS21000 4215350..4216276 - 927 WP_000003013.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
EW646_RS21005 4216324..4217433 - 1110 WP_001400431.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
EW646_RS21010 4217858..4218241 + 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
EW646_RS21015 4218238..4218765 - 528 WP_000342447.1 GNAT family N-acetyltransferase Toxin
EW646_RS21020 4218772..4219038 - 267 WP_001277109.1 DUF1778 domain-containing protein Antitoxin
EW646_RS21025 4219188..4220291 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
EW646_RS21030 4220562..4221416 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
EW646_RS21035 4221661..4222719 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
EW646_RS21040 4222712..4223380 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.63 Da        Isoelectric Point: 7.0284

>T118474 WP_000342447.1 NZ_CP035770:c4218765-4218238 [Escherichia coli O145]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDHSIQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T118474 NZ_CP035770:c4218765-4218238 [Escherichia coli O145]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTCACTCTTGGGCGTCTGGCAATTGATCATTCAATACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCAGTAGGTATTCACGGTCTTTTTGTAG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCGTTGGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9938.37 Da        Isoelectric Point: 10.0863

>AT118474 WP_001277109.1 NZ_CP035770:c4219038-4218772 [Escherichia coli O145]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AVKRLQGM

Download         Length: 267 bp

>AT118474 NZ_CP035770:c4219038-4218772 [Escherichia coli O145]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGTAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LUM2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LUN0

References